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| Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret, Jean-paul. |
| Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins... |
| Tipo: Text |
Palavras-chave: Microalgae; Biotechnology; Lipid; Nitrogen; Proteomic; Selection. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00179/29012/27470.pdf |
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| Zhu,Keming; Zhang,Weiwei; Sarwa,Rehman; Xu,Shuo; Li,Kaixia; Yang,Yanhua; Li,Yulong; Wang,Zheng; Cao,Jun; Li,Yaoming; Tan,Xiaoli. |
| Abstract Rapeseed is one of important oil crops in China. Better understanding of the regulation network of main agronomic traits of rapeseed could improve the yielding of rapeseed. In this study, we obtained an influrescence mutant that showed a fusion phenotype, similar with the Arabidopsis clavata-like phenotype, so we named the mutant as Bnclavata-like (Bnclv-like). Phenotype analysis illustrated that abnormal development of the inflorescence meristem (IM) led to the fused-inflorescence phenotype. At the stage of protein abundance, major regulators in metabolic processes, ROS metabolism, and cytoskeleton formation were seen to be altered in this mutant. These results not only revealed the relationship between biological processes and inflorescence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brassica napus; Proteomic; Inflorescence meristem (IM); Bnclavata-like (Bnclv-like); Quantitative real-time PCR. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100308 |
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| Chen,Xiao-Guang; Xu,Cun-Shuan. |
| BACKGROUND: Liver regeneration (LR) after 2/3 partial hepatectomy (PH) is one of the most studied models of cell, organ, and tissue regeneration. Although the transcriptional profile analysis of regenerating liver has been carried out by many reserachers, the dynamic protein expression profile during LR has been rarely reported up to date. Therefore, this study aims to detect the global proteomic profile of the regenerating rat liver following 2/3 hepatectomy, thereby gaining some insights into hepatic regeneration mechanism. RESULTS: Protein samples extracted from the sham-operated and the regenerating rat livers at 6, 12, 24, 72, 120 and 168 h after PH were separated by IEF/SDS-PAGE and then analyzed by MALDI-TOF/TOF mass spectrometry. Compared to... |
| Tipo: Journal article |
Palavras-chave: Partial hepatectomy; Rat liver regeneration; Proteomic; MALDI TOF/TOF MS. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100053 |
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| Song,Y.R.; Wu,B.; Yang,Y.T.; Chen,J.; Zhang,L.J.; Zhang,Z.W.; Shi,H.Y.; Huang,C.L.; Pan,J.X.; Xie,P.. |
| Bipolar disorder (BD) is a common psychiatric mood disorder affecting more than 1-2% of the general population of different European countries. Unfortunately, there is no objective laboratory-based test to aid BD diagnosis or monitor its progression, and little is known about the molecular basis of BD. Here, we performed a comparative proteomic study to identify differentially expressed plasma proteins in various BD mood states (depressed BD, manic BD, and euthymic BD) relative to healthy controls. A total of 10 euthymic BD, 20 depressed BD, 15 manic BD, and 20 demographically matched healthy control subjects were recruited. Seven high-abundance proteins were immunodepleted in plasma samples from the 4 experimental groups, which were then subjected to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bipolar disorder; Plasma; Proteomic; Carbonic anhydrase 1; Apolipoprotein. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100973 |
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| 滝柳, 泰文; 福田, 健二; 河原, 一樹; 三宅, 裕; 宮路, 淳子; 中澤, 隆; 常木, 晃; 平田, 昌弘; TAKIYANAGI, Yasufumi; FUKUDA, Kenji; KAWAHARA, Kazuki; MIYAKE, Yutaka; MIYAJI, Atsuko; NAKAZAWA, Takashi; TSUNEKI, Akira; HIRATA, Masahiro. |
| http://www.obihiro.ac.jp/~library/kenkyu.html |
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Palavras-chave: プロテオミクス; カゼイン; 古代土器片; 乳利用の起源; MALDI/TOF 質量分析装置; Proteomic; Casein; Ancient shard; Origin of milk use; MALDI/TOF mass spectrometry. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3818 |
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